Zap 70 (Ab 319) Antibody

Product Specifications

Background

ZAP70 is a 70-kD tyrosine phosphoprotein that associates with the ζ chain and undergoes tyrosine phosphorylation following TCR stimulation. The ZAP70 gene is expressed in T- and natural KILLER cells. Protein-Tyrosine Kinases (PTKs) play an integral role in T-cell activation. Stimulation of the T-cell antigen receptor results in tyrosine phosphorylation of a number of cellular substrates. One of these is the TCR-ζ chain, which can mediate the transduction of extracellular stimuli into cellular effector functions

NCBI Gene ID

7535

Swiss Prot

P43403

Accession Number

NP_001070.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Zap-70 (Ab-319) antibody was raised against a peptide sequence around aa.317~321 (S-P-Y-S-D) derived from Human ZAP70.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

70 kDa

Specificity

This antibody detects endogenous level of total Zap-70 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

ZAP70

NCBI Official Name

Zeta-chain (TCR) associated protein kinase 70kDa

NCBI Organism

Homo sapiens

Background Reference 01

Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100 (2) : 443-448Ku GM, et al. (2001) EMBO J; 20 (3) : 457-465Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96 (17) : 9775-9780Zhao Q, et al. (1996) Mol Cell Biol; 16 (12) : 6765-6774Williams BL, et al. (1999) EMBO J; 18 (7) : 1832-1844Adjali O, et al. (2005) J Clin Invest; 115 (8) : 2287-2295.