PAK1 / PAK2 / PAK3 (Ab 423 / 402 / 421) Antibody

Product Specifications

Background

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

NCBI Gene ID

5058;5062;5063

Swiss Prot

Q13153; ; Q13177; ; O75914

Accession Number

NP_001122092.1; NP_002568.2; NP_001121638.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

PAK1/PAK2/PAK3 (Ab-423/402/421) antibody was raised against a peptide sequence around aa.421~425/400~404/419~423 (R-S-T-M-V) derived from Human PAK1/PAK2/PAK3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

61 - 67 (PAK2) 68 - 74 (PAK1/3) kDa

Specificity

This antibody detects endogenous level of total PAK1/PAK2/PAK3 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PAK1 PAK2 PAK3

NCBI Official Name

P21 protein (Cdc42/Rac) -activated kinase 1 / p21 protein (Cdc42/Rac) -activated kinase 2 / p21 protein (Cdc42/Rac) -activated kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695. Jung JH, et al. (2005) J Biol Chem; 280:40025-40031. Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.