FAK (Ab 925) Antibody

Product Specifications

Background

Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

NCBI Gene ID

5747

Swiss Prot

Q05397

Accession Number

NP_005598.3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

FAK (Ab-925) antibody was raised against a peptide sequence around aa.923~927 (K-V-Y-E-N) derived from Human FAK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

125 kDa

Specificity

This antibody detects endogenous level of total FAK protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

PTK2

NCBI Official Name

Protein tyrosine kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Sanders MA, et al. (2005) J Biol Chem; 280 (25) : 23516-22. Cherubini A, et al. (2005) Mol Biol Cell; 16 (6) : 2972-83. Toriumi Y, et al. (2003) FEBS Lett; 553 (3) : 419-22. Shi Q, et al. (2003) Mol Biol Cell; 14 (10) : 4306-15.