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HDAC5 (Ab 498) Antibody

Product Specifications

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by HDAC5 belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.

NCBI Gene ID

10014

Swiss Prot

Q9UQL6

Accession Number

NP_001015053.1

Host

Rabbit

Reactivity

Human

Immunogen

HDAC5 (Ab-498) antibody was raised against a peptide sequence around aa.496~500 (T-Q-S-S-P) derived from Human HDAC5/7.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

124 kDa

Specificity

This antibody detects endogenous level of total HDAC5 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

HDAC5

NCBI Official Name

Histone deacetylase 5

NCBI Organism

Homo sapiens

Background Reference 01

Doppler H, et al. (2005) J Biol Chem. 280 (15) : 15013-15019. McKinsey TA, et al. (2000) Nature. 408 (6808) : 106-111.

Other Product Names

HD5, NY-CO-9, CYK18, PIG46, HD5

Tested Applications

WB, IHC

Protein ID

62750349

Physical Properties

Liquid

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