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MKK3 (Ab 189) Antibody

Product Specifications

Background

MEK3 belongs to MAPKK family. This kinase is activated by mitogenic and environmental stress, and participates in the MAPK-mediated signaling cascade. It phosphorylates and thus activates p38. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of Ras oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of p38, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis.

NCBI Gene ID

5606

Swiss Prot

P46734

Accession Number

NP_002747.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MKK3 (Ab-189) antibody was raised against a peptide sequence around aa.187~191 (V-D-S-V-A) derived from Human MKK3.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 kDa

Specificity

This antibody detects endogenous level of total MKK3 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

MAP2K3

NCBI Official Name

Mitogen-activated protein kinase kinase 3

NCBI Organism

Homo sapiens

Background Reference 01

Wang W, et al. (2002) Mol Cell Biol ; 22 (10) : 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16 (3) : 1247-55.

Other Product Names

MEK3, MKK3, MAPKK3, PRKMK3, SAPKK2, SAPKK-2, ERK1, PRKM3, MAP kinase kinase 3, MAP2K3, MAPK/ERK kinase 3, MAPKK 3, MEK3

Tested Applications

WB, IHC, IF

Protein ID

21618347

Physical Properties

Liquid

Frequently Asked Questions

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