Chk1 (Ab 317) Antibody

Product Specifications

Background

Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90% sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29%, 40%, and 44% identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry

NCBI Gene ID

1111

Swiss Prot

O14757

Accession Number

NP_001107593.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Chk1 (Ab-317) antibody was raised against a peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

56 kDa

Specificity

This antibody detects endogenous level of total Chk1 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CHEK1

NCBI Official Name

Checkpoint kinase 1

NCBI Organism

Homo sapiens

Background Reference 01

Zhang YW, et al. (2005) Mol Cell; 19 (5) : 607-18. Bhoumik A, et al. (2005) Mol Cell; 18 (5) : 577-87. Rocha S, et al. (2005) EMBO J. Clarke CA, et al. (2005) Biochem J. Yu X, et al. (2004) Mol Cell Biol; 24 (21) : 9478-86.