Rb (Ab 807) Antibody

Product Specifications

Background

Rb operates in the midst of the cell cycle clock apparatus. Its main role is to act as a signal transducer connecting the cell cycle clock with the transcriptional machinery (Ref.1) . It plays an important role in the Rb/ E2F pathway in cell proliferation, cell fate determination, and cancer (Ref.2) . Cellular senescence is a stable form of cell cycle arrest that limits proliferation of damaged cells and act as a natural barrier to cancer progression. A distinct heterochromatic structure that accumulates in senescent human fibroblasts, designated as SAHF (Senescence-Associated Heterochromatic Foci) . SAHF formation coincides with recruitment of heterochromatin proteins and the Rb protein to E2F-responsive promoters and is associated with the stable repression of E2F target genes. Both SAHF formation and the silencing of E2F target genes depended on the integrity of the Rb pathway and do not occur in reversibly arrested cells (Ref.3) . Rb activates transcription of the c-Jun gene through the SP1-binding site within the c-Jun promoter

NCBI Gene ID

5925

Swiss Prot

P06400

Accession Number

NP_000312.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Rb (Ab-807) antibody was raised against a peptide sequence around aa.805~809 (Y-I-S-P-L) derived from Human Rb.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

110 kDa

Specificity

This antibody detects endogenous level of total Rb protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

RB1

NCBI Official Name

Retinoblastoma 1

NCBI Organism

Homo sapiens

Background Reference 01

Roesch A, et al. (2005) Mod Pathol. 18 (4) : 565-572. Chadee DN, et al. (2004) Nat Cell Biol. 6 (8) : 770-776. Knudsen ES, et al. (1997) Mol Cell Biol. 17 (10) : 5771-5783. Knudsen ES, et al. (1996) J Biol Chem. 271 (14) : 8313-8320.