EGFR (Ab 1070) Antibody

Product Specifications

Background

Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentia

NCBI Gene ID

1956

Swiss Prot

P00533

Accession Number

NP_005219.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

EGFR (Ab-1070) antibody was raised against a peptide sequence around aa.1068~1072 (R-Y-S-S-D) derived from Human EGFR.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

175 kDa

Specificity

This antibody detects endogenous level of total EGFR protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

EGFR

NCBI Official Name

Epidermal growth factor receptor

NCBI Organism

Homo sapiens

Background Reference 01

Feinmesser RL, et al. (1999) J Biol Chem; 274 (23) : 16168-73. Gamou S, et al. (1995) FEBS Lett; 357 (2) : 161-4. Gamou S, et al. (1994) J Cell Physiol; 158 (1) : 151-9. Heisermann GJ, et al. (1988) J Biol Chem; 263 (26) : 13152-8.