HER2 (Ab 1248) Antibody

Product Specifications

Background

Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Not activated by EGF, TGF-alpha and amphiregulin.

NCBI Gene ID

2064

Swiss Prot

P04626

Accession Number

NP_001005862.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HER2 (Ab-1248) antibody was raised against a peptide sequence around aa. 1246~1250 (P-E-Y-L-G) derived from Human HER2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

185 kDa

Specificity

This antibody detects endogenous level of total HER2 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

ERBB2

NCBI Official Name

V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2

NCBI Organism

Homo sapiens

Background Reference 01

Ramsauer VP, et al. (2003) J Biol Chem; 278 (32) : 30142-7. Jepson S, et al. (2002) Oncogene; 21 (49) : 7524-32. Ren Z, et al. (2002) J Biol Chem; 277 (41) : 38486-93. Eppenberger-Castori S, et al. (2001) J Clin Oncol; 19 (3) : 645-56.