BCL 2 (Ab 70) Antibody

Product Specifications

Background

BCL-2 encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

NCBI Gene ID

596

Swiss Prot

P10415

Accession Number

NP_000624.2

Host

Rabbit

Reactivity

Human

Immunogen

BCL-2 (Ab-70) antibody was raised against a peptide sequence around aa.68~72 (R-T-S-P-L) derived from Human BCL-2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

26 kDa

Specificity

This antibody detects endogenous level of total BCL-2 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

BCL2

NCBI Official Name

B-cell CLL/lymphoma 2

NCBI Organism

Homo sapiens

Background Reference 01

Muller IM, et al. (2005) Mol Pharmacol. Jin Z, Gao F, et al. J Biol Chem 2004 Sep 17; 279 (38) : 40209-19. Kumar Biswas S, et al. Mol Cancer Ther 2004 Mar; 3 (3) : 327-34. Huang ST, et al. (2002) FASEB J; 16 (8) : 825-32. Yamamoto K, et al. (1999) Mol Cell Biol; 19 (12) : 8469-78.