BCL 2 (Ab 56) Antibody

Product Specifications

Background

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) .

NCBI Gene ID

596

Swiss Prot

P10415

Accession Number

NP_000624.2

Host

Rabbit

Reactivity

Human

Immunogen

BCL-2 (Ab-56) antibody was raised against a peptide sequence around aa. 54~58 (G-H-T-P-H) derived from Human BCL-2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

26 kDa

Specificity

This antibody detects endogenous level of total BCL-2 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

BCL2

NCBI Official Name

B-cell CLL/lymphoma 2

NCBI Organism

Homo sapiens

Background Reference 01

Ling, Y. H. et al. (1998) J. Biol. Chem. 273, 18984-18991. Huang, S. J. and Cidlowski, J. A. (2002) FASEB 16, 825-832. Deng, X. et al. (2001) J. Biol. Chem. 276, 23681-23688. Huang ST, et al. (2002) FASEB J Jun; 16 (8) : 825-32. Yamamoto, K. et al. (1999) Mol. Cell. Biol. 19, 8469-8478.