What is the catalog number for Akt (Ab 308) Antibody?

The catalog number for Akt (Ab 308) Antibody is 79-295.

What sizes are available for Akt (Ab 308) Antibody?

Akt (Ab 308) Antibody is available in the following size(s): 0.1 mg.

Is Akt (Ab 308) Antibody in stock?

Akt (Ab 308) Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Akt (Ab 308) Antibody

Product Specifications

Background

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

NCBI Gene ID

207

Swiss Prot

P31749

Accession Number

NP_001014431.1

Host

Rabbit

Reactivity

Human

Immunogen

Akt (Ab-308) antibody was raised against a peptide sequence around aa.306~310 (M-K-T-F-C) derived from Human AKT1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of total Akt protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

AKT1

NCBI Official Name

V-akt murine thymoma viral oncogene homolog 1

NCBI Organism

Homo sapiens

Background Reference 01

Tremblay F, et al. (2005) Diabetes; 54 (9) : 2674-84. Xu BE, et al. (2005) J Biol Chem; 280 (40) : 34218-23. Samuels Y, et al. (2005) Cancer Cell; 7 (6) : 561-73. Di Maira G, et al. (2005) Cell Death Differ; 12 (6) : 668-77.