CREB (Ab 133) Antibody

Product Specifications

Background

This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

NCBI Gene ID

1385

Swiss Prot

P16220

Accession Number

NP_004370.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CREB (Ab-133) antibody was raised against a peptide sequence around aa. 131~135 (R-P-S-Y-R) derived from Human CREB.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Specificity

This antibody detects endogenous level of total CREB protein and it also detects the CREB-related protein ATF-1.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CREB1

NCBI Official Name

CAMP responsive element binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Xing J, et al. (1998) Mol Cell Biol 18 (4) : 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17) : 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27) : 25184-9. Lu, H. et al. (1997) Mol. Cell. Biol. 17, 5923-5934.