Myc (Ab 58) Antibody

Product Specifications

Background

Myc proto-oncogene encodes nuclear DNA-binding phosphoproteins that are involved in the regulation of gene expression and DNA replication during cell growth and differentiation. Myc encodes a protein of 65 kDa which is expressed in almost all normal and transformed cells. The expression correlates with the proliferation state of the cells. Transcription is repressed in quiescent or terminally differentiated cells. Expression of Myc is generally induced after mitogenic stimulation of cells or serum induction. Myc therefore is an important positive regulator of cell growth and proliferation. Myc has been demonstrated also to be a potent inducer of apoptosis when expressed in the absence of serum or growth factors. Apoptosis may serve also as a protective mechanism to prevent tumorigenicity elicited by deregulated Myc expression. Sequences of the Myc oncogene have been highly conserved throughout evolution, from Drosophila to vertebrates

NCBI Gene ID

4609

Swiss Prot

P01106

Accession Number

NP_002458.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Myc (Ab-58) antibody was raised against a peptide sequence around aa.56~60 (L-P-T-P-P) derived from Human Myc.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of total Myc protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MYC

NCBI Official Name

V-myc avian myelocytomatosis viral oncogene homolog

NCBI Organism

Homo sapiens

Background Reference 01

Jin Z, et al. (2004) J Biol Chem. 279 (38) : 40209-40219. Welcker M, et al. (2004) Proc Natl Acad Sci U S A. 101 (24) : 9085-9090. Baudino T A, et al. (2001) Mol Cell Biol. 21: 691-702. Blackwood E M, et al. (1991) Science. 251:1211-1217. Henriksson M, et al. (1996) Adv Cancer Res. 68: 109-182.