MEK 2 (Ab 394) Antibody

Product Specifications

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases

NCBI Gene ID

5605

Swiss Prot

P36507

Accession Number

NP_109587.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MEK-2 (Ab-394) antibody was raised against a peptide sequence around aa.392~396 (P-G-T-P-T) derived from Human MEK-2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Other

Purification

Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

None

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

44 kDa

Specificity

This antibody detects endogenous level of total MEK2 protein.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAP2K2

NCBI Official Name

Mitogen-activated protein kinase kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852.