C Cbl (phospho Tyr700) Antibody
Product Specifications
Background
Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.
NCBI Gene ID
867
Swiss Prot
P22681
Accession Number
NP_005179.2
Host
Rabbit
Reactivity
Human
Immunogen
C-Cbl (phospho-Tyr700) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 770 (T-E-Y (p) -M-T) derived from Human c-Cbl.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Apoptosis, Cancer
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
120 kDa
Specificity
This antibody detects endogenous level of c-Cbl only when phosphorylated at tyrosine 770.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
CBL
NCBI Official Name
Cbl proto-oncogene, E3 ubiquitin protein ligase
NCBI Organism
Homo sapiens
Background Reference 01
Blake, T. J. et al. (1991) Oncogene 6, 653-657. Thien, C. B. and Langdon, W. Y. (1998) Immunol. Cell Biol. 76, 473-482. Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339. Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
Other Product Names
CBL2, NSLL, C-CBL, RNF55, FRA11B, NR3C3, Signal transduction protein CBL, Proto-oncogene c-CBL, Casitas B-lineage lymphoma proto-oncogene, RING finger protein 55
Tested Applications
WB, IHC, IF
Protein ID
52426745
Physical Properties
Liquid
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