CDK6 (phospho Tyr24) Antibody

Product Specifications

Background

Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.

NCBI Gene ID

1021

Swiss Prot

Q00534

Accession Number

NP_001138778.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

CDK6 (phospho-Tyr24) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y (p) -G-K) derived from Human CDK6.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

36 kDa

Specificity

This antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 24.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

CDK6

NCBI Official Name

Cyclin-dependent kinase 6

NCBI Organism

Homo sapiens

Background Reference 01

Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659. Pestell, R. G. et al. (1999) Endocr. Rev. 20, 501-534. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625.