Cyclin B1 (phospho Ser147) Antibody
Product Specifications
Background
The protein encoded by Cyclin B1 is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF) . Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
NCBI Gene ID
891
Swiss Prot
P14635
Accession Number
NP_114172.1
Host
Rabbit
Reactivity
Human
Immunogen
Cyclin B1 (phospho-Ser147) antibody was raised against a peptide sequence around phosphorylation site of Serine 147 (A-F-S (p) -D-V) derived from Human Cyclin B1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Cell Cycle
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
60 kDa
Specificity
This antibody detects endogenous level of Cyclin B1 only when phosphorylated at serine 147.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
CCNB1
NCBI Official Name
Cyclin B1
NCBI Organism
Homo sapiens
Background Reference 01
Norbury, C. and Nurse, P. (1992) Annu. Rev. Biochem. 61, 441-470. Atherton-Fessler, S. et al. (1993) Mol. Cell. Biol. 13, 1675-1685. Galaktionov, K. et al. (1995) Genes Dev. 9, 1046-1058.
Other Product Names
CCNB, VEGFR3
Tested Applications
WB, IHC, IF
Protein ID
14327896
Physical Properties
Liquid
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