MARCKS (phospho Ser170) Antibody

Product Specifications

Background

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

NCBI Gene ID

4082

Swiss Prot

P29966

Accession Number

NP_002347.5

Host

Rabbit

Reactivity

Human

Immunogen

MARCKS (phospho-Ser170) antibody was raised against a peptide sequence around phosphorylation site of Serine 170 (G-F-S (p) -F-K) derived from Human MARCKS.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

80 kDa

Specificity

This antibody detects endogenous level of MARCKS only when phosphorylated at threonine 170.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MARCKS

NCBI Official Name

Myristoylated alanine-rich protein kinase C substrate

NCBI Organism

Homo sapiens

Background Reference 01

Ramsden, J. J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479. Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823. Hartwig, J. H. et al. (1992) Nature 356, 618-622. Thelen, M. et al. (1991) Nature 351, 320-322.