HDAC4 / HDAC5 / HDAC9 (phospho Ser246 / Ser259 / Ser220) Antibody

Product Specifications

Background

Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general, they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol) . HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.

NCBI Gene ID

9759;10014;9734

Swiss Prot

P56524Q9UQL6Q9UKV0

Accession Number

NP_006028.2; NP_001015053.1; NP_055522.1

Host

Rabbit

Reactivity

Human

Immunogen

HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody was raised against a peptide sequence around phosphorylation site of serine 246/259/220 (T-A-S (p) -EP) derived from Human HDAC4/HDAC5/HDAC9.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

140, 124 kDa

Specificity

This antibody detects endogenous level of HDAC4/HDAC5/HDAC9 only when phosphorylated at serine 246/259/220.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

HDAC4 HDAC5 HDAC9

NCBI Official Name

Histone deacetylase 4 / histone deacetylase 5 / histone deacetylase 9

NCBI Organism

Homo sapiens

Background Reference 01

Cress, W. D. and Seto, E. (2000) J Cell Physiol 184, 1-16. Vigushin, D. M. and Coombes, R. C. (2004) Curr. Cancer Drug Targets 4, 205-218. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100.