EIF4B (phospho Ser422) Antibody
Product Specifications
Background
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
NCBI Gene ID
1975
Swiss Prot
P23588
Accession Number
NP_001408.2
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
EIF4B (phospho-Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (T-G-S (p) -E-S) derived from Human eIF4B.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=79-242
Calculated Molecular Weight
80 kDa
Specificity
This antibody detects endogenous level of eIF4B only when phosphorylated at Serine 422.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
EIF4B
NCBI Official Name
Eukaryotic translation initiation factor 4B
NCBI Organism
Homo sapiens
Background Reference 01
Gingras, A. C. et al. (2001) Genes Dev. 15, 807-826. Duncan, R. and Hershey, J. W. (1985) J. Biol. Chem. 260, 5493-5497. Duncan, R. F. and Hershey, J. W. (1989) J. Cell Biol. 109, 1467-1481.
Other Product Names
EIF-4B, PRO1843, ESR, NR3A1
Tested Applications
WB, IHC
Protein ID
50053795
Physical Properties
Liquid
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