Cofilin1 / cofilin2 (phospho Tyr88) Antibody

Product Specifications

Background

Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

NCBI Gene ID

1072;1073

Swiss Prot

P23528Q9Y281

Accession Number

NP_005498.1; NP_068733.1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Cofilin1/cofilin2 (phospho-Tyr88) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 88 (A-T-Y (p) -E-T) derived from Human coflin1/cofilin2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

19 kDa

Specificity

This antibody detects endogenous level of cofilin1/cofilin2 only when phosphorylated at tyrosine 88.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CFL1 CFL2

NCBI Official Name

Cofilin 1 (non-muscle) / cofilin 2 (muscle)

NCBI Organism

Homo sapiens

Background Reference 01

Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830. Arber, S. et al. (1998) Nature 393, 805-809. Yang, N. et al. (1998) Nature 393, 809-812.

Other Product Names

CFL, HEL-S-15, ESR, NR3A1, CFL1/CFL2

Tested Applications

IHC, WB

Protein ID

5031635 / 14719392

Physical Properties

Liquid

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