AKT1 (phospho Thr450) Antibody

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I) . Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.

AKT1 (phospho-Thr450) antibody was raised against a peptide sequence around phosphorylation site of threonine 450 (T-I-T (p) -P-P) derived from Human AKT1.

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Xing J, et al. (1998) Mol Cell Biol 18 (4) : 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17) : 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27) : 25184-9.