Smad3 (phospho Ser425) Antibody
Product Specifications
Background
Smad3 encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.
NCBI Gene ID
4088
Swiss Prot
P84022
Accession Number
NP_001138574.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Smad3 (Phospho-Ser425) antibody was raised against a peptide sequence around phosphorylation site of serine 425 (C-S-S-V-S (p) ) derived from Human Smad3.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
52 kDa
Specificity
This antibody detects endogenous level of Smad3 only when phosphorylated at serine 425.
Applications Notes
Immunohistochemistry: 1:50~1:100
Symbol
SMAD3
NCBI Official Name
SMAD family member 3
NCBI Organism
Homo sapiens
Background Reference 01
Shi W, et al. J Cell Sci. 2007 Apr 1;120 (Pt 7) : 1216-24Seong HA, et al. J Biol Chem. 2007 Apr 20;282 (16) : 12272-89Wordinger RJ, et al. Invest Ophthalmol Vis Sci. 2007 Mar;48 (3) : 1191-200LeClair RJ, et al. Circ Res. 2007 Mar 30;100 (6) : 826-33
Other Product Names
LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436, PTP2C, SHPTP2, MAD-3, Mad3, Mothers against DPP homolog 3
Tested Applications
IHC
Protein ID
223029440
Physical Properties
Liquid
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