SHP 2 (phospho Tyr542) Antibody
Product Specifications
Background
Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
NCBI Gene ID
5781
Swiss Prot
Q06124
Accession Number
NP_002825.3
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
SHP-2 (Phospho-Tyr542) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 542 (H-E-Y (p) -T-N) derived from Human SHP-2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
72 kDa
Specificity
This antibody detects endogenous level of SHP-2 only when phosphorylated at tyrosine 542.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
PTPN11
NCBI Official Name
Protein tyrosine phosphatase, non-receptor type 11
NCBI Organism
Homo sapiens
Background Reference 01
Ferjoux G, et al. (2003) Mol Biol Cell. 2003; 14 (9) : 3911-3928. Shi ZQ, et al. (2000) Mol Cell Biol. 20 (5) : 1526-1536. Li C, Friedman JM. (1999) Proc Natl Acad Sci U S A ; 96 (17) : 9677-9682Manes S, et al. (1999) Mol Cell Biol. 19 (4) : 3125-3135. Oh ES, et al. (1999) Mol Cell Biol; 19 (4) : 3205-3215.
Other Product Names
CFC, NS1, SHP2, BPTP3, PTP2C, PTP-1D, SH-PTP2, SH-PTP3, FRAP, FRAP1, FRAP2, RAFT1, RAPT1, PTN11, PTP-2C, PTPN11
Tested Applications
WB, IHC
Protein ID
33356177
Physical Properties
Liquid
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