NFkB p105 / p50 (phospho Ser927) Antibody

Product Specifications

Background

NF-κ -B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ -B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

NCBI Gene ID

4790

Swiss Prot

P19838

Accession Number

NP_001158884.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

NFkB-p105/p50 (Phospho-Ser927) antibody was raised against a peptide sequence around phosphorylation site of serine 927 (C-D-S (p) -G-V) derived from Human NFκ B-p105.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

50, 120 kDa

Specificity

This antibody detects endogenous level of NFκ B-p105/p50 only when phosphorylated at serine 927.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

NFKB1

NCBI Official Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1

NCBI Organism

Homo sapiens

Background Reference 01

Hou S, et al. (2003) J Biol Chem. 278 (46) : 45994-45998. Baeuerle P A, et al. (1994) Annu Rev Immunol. 12:141-179. Baeuerle P A, et al. (1996) Cell 87:13-20. Haskill S, et al. (1991) Cell 65:1281-1289.

Other Product Names

P50, KBF1, p105, EBP-1, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa-B, SHC, SHCA, DNA-binding factor KBF1, NF-kappa-B1 p84/NF-kappa-B1 p98, NFKB1, NFkB-p50