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Keratin 18 (phospho Ser33) Antibody

Product Specifications

Background

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. utations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

NCBI Gene ID

3875

Swiss Prot

P05783

Accession Number

NP_000215.1

Host

Rabbit

Reactivity

Human

Immunogen

Keratin 18 (Phospho-Ser33) antibody was raised against a peptide sequence around phosphorylation site of serine 33 (A-A-S (p) -V-Y) derived from Human Keratin 18 (CK18) .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

46 kDa

Specificity

This antibody detects endogenous level of Keratin 18 only when phosphorylated at serine 33.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

KRT18

NCBI Official Name

Keratin 18

NCBI Organism

Homo sapiens

Background Reference 01

Ramaekers, F. C. and Bosman, F. T. (2004) J. Pathol. 204, 351-354. Chang, L. and Goldman, R. D. (2004) Nat. Rev. Mol. Cell Biol. 5, 601-613. Moll, R. et al. (1982) Cell 31, 11-24.

Other Product Names

K18, CYK18, CBL2, RNF55, CK 18, CK18, Cytokeratin endo B, K18

Tested Applications

WB, IHC

Protein ID

4557888

Physical Properties

Liquid

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