IkB beta (phospho Ser23) Antibody

Product Specifications

Background

Inhibits NF-κ -B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-κ -B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

NCBI Gene ID

4793

Swiss Prot

Q15653

Accession Number

NP_001001716.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IkB-beta (Phospho-Ser23) antibody was raised against a peptide sequence around phosphorylation site of serine 23 (L-G-S (p) -L-G) derived from Human IkappaB-β .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

48 kDa

Specificity

This antibody detects endogenous level of IkappaB-β only when phosphorylated at serine 23.

Applications Notes

Immunohistochemistry: 1:50~1:100

Symbol

NFKBIB

NCBI Official Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta

NCBI Organism

Homo sapiens

Background Reference 01

Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174. DiDonato J, et al. (1996) Mol Cell Biol 16 (4) : 1295-304

Other Product Names

IKBB, TRIP9, HNF4, NR2A1, TCF14, I-kappa-B-beta, IKB-B, IKBB, NF-kappa-BIB, NF-kappaB inhibitor beta

Tested Applications

IHC

Protein ID

48928017

Physical Properties

Liquid

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