GSK3beta (phospho Tyr216) Antibody
Product Specifications
Background
Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.
NCBI Gene ID
2932
Swiss Prot
P49841
Accession Number
NP_001139628.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
GSK3beta (Phospho-Tyr216) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 216 (V-S-Y (p) -I-C) derived from Human GSK3β .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Cell Cycle
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
46 kDa
Specificity
This antibody detects endogenous level of GSK3β only when phosphorylated at tyrosine 216.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
GSK3B
NCBI Official Name
Glycogen synthase kinase 3 beta
NCBI Organism
Homo sapiens
Background Reference 01
Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137 (1-2) : 193-201Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316 (2) : 411-415Fan G, et al. J Biol Chem 2003 Dec 26; 278 (52) : 52432-52436Liao X, et al. Mol Cancer Ther 2003 Nov; 2 (11) : 1215-1222
Other Product Names
FLK1, VEGFR2, Factor A, GSK-3 alpha/beta, kinase GSK3-alpha/beta
Tested Applications
WB, IHC, IF
Protein ID
225903437
Physical Properties
Liquid
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