GSK3beta (phospho Tyr216) Antibody

Product Specifications

Background

Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin.

NCBI Gene ID

2932

Swiss Prot

P49841

Accession Number

NP_001139628.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

GSK3beta (Phospho-Tyr216) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 216 (V-S-Y (p) -I-C) derived from Human GSK3β .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

46 kDa

Specificity

This antibody detects endogenous level of GSK3β only when phosphorylated at tyrosine 216.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

GSK3B

NCBI Official Name

Glycogen synthase kinase 3 beta

NCBI Organism

Homo sapiens

Background Reference 01

Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137 (1-2) : 193-201Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316 (2) : 411-415Fan G, et al. J Biol Chem 2003 Dec 26; 278 (52) : 52432-52436Liao X, et al. Mol Cancer Ther 2003 Nov; 2 (11) : 1215-1222