PKCdelta (phospho Ser645) Antibody
Product Specifications
Background
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
NCBI Gene ID
5580
Swiss Prot
Q05655
Accession Number
NP_006245.2
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
PKCdelta (Phospho-Ser645) antibody was raised against a peptide sequence around phosphorylation site of serine 645 (R-L-S (p) -Y-S) derived from Human PKCδ .
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Apoptosis, Cancer, Neuroscience
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
78 kDa
Specificity
This antibody detects endogenous level of PKCδ only when phosphorylated at serine 645.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
PRKCD
NCBI Official Name
Protein kinase C, delta
NCBI Organism
Homo sapiens
Background Reference 01
Kei Sakamoto, et, al. (2003) Am J Physiol Endocrinol Metab ; 285: E1081 - E1088. Ling Zhang, et, al. (2004) J. Biol. Chem ; 279: 28315 - 28319. Kristof Van Kolen et, al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et, al. (2002) J. Cell Biol ; 157: 253.
Other Product Names
MAY1, PKCD, CVID9, nPKC-delta, BHLHC1, MYF3, MYOD, KPCD, PKC-delta, PRKCD, kinase PKC-delta
Tested Applications
WB, IHC
Protein ID
31377782
Physical Properties
Liquid
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