PKR (phospho Thr446) Antibody

Product Specifications

Background

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection.

NCBI Gene ID

5610

Swiss Prot

P19525

Accession Number

NP_001129123.1

Host

Rabbit

Reactivity

Human

Immunogen

PKR (Phospho-Thr446) antibody was raised against a peptide sequence around phosphorylation site of threonine 446 (K-R-T (p) -R-S) derived from Human PKR.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

68 kDa

Specificity

This antibody detects endogenous level of PKR only when phosphorylated at threonine 446.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

EIF2AK2

NCBI Official Name

Eukaryotic translation initiation factor 2-alpha kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Abujiang Pataer, et, al. (2002) Cancer Res; 62: 2239. K. D. Ryman, et, al. (2005) J. Virol; 79: 1487 - 1499. Susana Guerra, et, al. (2006) J. Biol. Chem; 281: 18734 - 18745.