P56Dok 2 (phospho Tyr299) Antibody

Product Specifications

Background

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation

NCBI Gene ID

9046

Swiss Prot

O60496

Accession Number

NP_003965.2

Host

Rabbit

Reactivity

Human

Immunogen

P56Dok-2 (Phospho-Tyr299) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 299 (G-E-Y (p) -A-V) derived from Human p56Dok-2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

56 kDa

Specificity

This antibody detects endogenous level of p56Dok-2 only when phosphorylated at tyrosine 299.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

DOK2

NCBI Official Name

Docking protein 2, 56kDa

NCBI Organism

Homo sapiens

Background Reference 01

Feng Cong, et, al. (1999) Mol. Cell. Biol ; 19: 8314 - 8325. Serge Lemay, et, al. (2000) Mol. Cell. Biol ; 20: 2743 - 2754. Ute Schaeper, et, al. (2000) J. Cell Biol ; 149: 1419. Miyuki Honma, et, al. (2006) Genes Cells; 11: 143 - 151.