P62Dok (phospho Tyr398) Antibody

Product Specifications

Background

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK1 appears to be a negative regulator of the insulin signaling pathway. Modulates integrin activation by competing with talin for the same binding site on ITGB3.

NCBI Gene ID

1796

Swiss Prot

Q99704

Accession Number

NP_001372.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P62Dok (phospho-Tyr398) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 398 (E-G-Y (p) -E-L) derived from Human p62Dok.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

62 kDa

Specificity

This antibody detects endogenous level of p62Dok only when phosphorylated at tyrosine 398.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

DOK1

NCBI Official Name

Docking protein 1, 62kDa (downstream of tyrosine kinase 1)

NCBI Organism

Homo sapiens

Background Reference 01

Michael J. Wick, et al. (2001) J. Biol. Chem ; 276: 42843 - 42850. Paul D. Simoncic, et al. (2006) Mol. Cell. Biol ; 26: 4149 - 4160. Nadia Dube, et al. (2004) PNAS ; 101: 1834. Adam C. Yopp, et al. (2005) J. Immunol ; 175: 2913 - 2924.