CREB (phospho Ser129) Antibody

Product Specifications

Background

This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.

NCBI Gene ID

1385

Swiss Prot

P16220

Accession Number

NP_004370.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

CREB (Phospho-Ser129) antibody was raised against a peptide sequence around phosphorylation site of serine 129 (I-L-S (p) -R-R) derived from Human CREB.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer, Neuroscience

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Specificity

This antibody detects endogenous level of CREB only when phosphorylated at serine 129.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CREB1

NCBI Official Name

CAMP responsive element binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Chrystelle V. Garat, et al. (2006) Mol. Cell. Biol ; 26: 4934 - 4948. Lilah Rothem, et al. (2004) Mol. Pharmacol ; 66: 1536 - 1543. Darren R. Tyson, et, al. (2002) Endocrinology; 143: 674. Kyung-Woo Park, et al. (2003) Arterioscler. Thromb. Vasc. Biol ; 23: 1364.