P70 S6 Kinase (phospho Ser411) Antibody

Product Specifications

Background

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.

NCBI Gene ID

6198

Swiss Prot

P23443

Accession Number

NP_003152.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P70 S6 Kinase (Phospho-Ser411) antibody was raised against a peptide sequence around phosphorylation site of serine 411 (I-R-S (p) -P-R) derived from Human p70 S6 Kinase.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

70 85 kDa

Specificity

This antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 411.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

RPS6KB1

NCBI Official Name

Ribosomal protein S6 kinase, 70kDa, polypeptide 1

NCBI Organism

Homo sapiens

Background Reference 01

Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24) : 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97