RelB (phospho Ser573) Antibody
Product Specifications
Background
NF-κ -B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-κ -B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-κ -B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-κ -B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-κ -B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-κ -B complex which translocates to the nucleus. NF-κ -B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49.
NCBI Gene ID
5971
Swiss Prot
Q01201
Accession Number
NP_006500.2
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
RelB (Phospho-Ser573) antibody was raised against a peptide sequence around phosphorylation site of serine 573 (L-L-S (p) -P-G) derived from Human RelB.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Product Datasheet
https://www.prosci-inc.com/?datasheet_sku=79-220
Calculated Molecular Weight
70 kDa
Specificity
This antibody detects endogenous level of RelB only when phosphorylated at serine 573.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
RELB
NCBI Official Name
V-rel avian reticuloendotheliosis viral oncogene homolog B
NCBI Organism
Homo sapiens
Background Reference 01
Marienfeld R, et al. (2001) Oncogene. 20 (56) : 8142-7. Charlotte S. Kaetzel1, et al. (2005) Immunological Reviews Volume 206: 83 Elwira Pyz, et al. (2006) J Immunol. 176:7447-55
Other Product Names
IREL, I-REL, REL-B, PDK1, I-Rel
Tested Applications
WB, IHC
Protein ID
35493878
Physical Properties
Liquid
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
