P38 MAPK (phospho Thr180) Antibody

Product Specifications

Background

Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.

NCBI Gene ID

1432

Swiss Prot

Q16539

Accession Number

NP_001306.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P38 MAPK (Phospho-Thr180) antibody was raised against a peptide sequence around phosphorylation site of threonine 180 (E-M-T (p) -G-Y) derived from Human P38MAPK.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

43 kDa

Specificity

This antibody detects endogenous level of P38 MAPK only when phosphorylated at Threonine 180.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAPK14

NCBI Official Name

Mitogen-activated protein kinase 14

NCBI Organism

Homo sapiens

Background Reference 01

Kim JE, et al. (2005) J Proteome Res Jul-Aug; 4 (4) : 1339-1346Meng F, et al. (2005) Am J Physiol Cell Physiol May 25Jin ZH, et al. (2005) Oncogene Mar 17; 24 (12) : 1973-1981