HSP27 (phospho Ser78) Antibody

Product Specifications

Background

Involved in stress resistance and actin organization.

NCBI Gene ID

3315

Swiss Prot

P04792

Accession Number

NP_001531.1

Host

Rabbit

Reactivity

Human

Immunogen

HSP27 (Phospho-Ser78) antibody was raised against a peptide sequence around phosphorylation site of serine 78 (A-L-S (p) -R-Q) derived from Human HSP27.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

27 kDa

Specificity

This antibody detects endogenous level of HSP27 only when phosphorylated at serine 78.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

HSPB1

NCBI Official Name

Heat shock 27kDa protein 1

NCBI Organism

Homo sapiens

Background Reference 01

J Landry, et al. (1992) J. Biol. Chem., Vol. 267, Issue 2: 794-803Sita Somara, et al. (2004) Am J Physiol Cell Physiol 286: C1290-C1301Catherine Gaitanaki, et al. (2003) Experimental Biology 206, 2759-2769 (2003) Jing Dong, et al. (2004) Am J Physiol Renal Physiol 287: F1049-F1058

Other Product Names

CMT2F, HMN2B, HSP27, HSP28, Hsp25, SRP27, HS.76067, HEL-S-102, KIAA0204, STK2, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Growth-related 25 kDa protein, HSP 27, HSP25