P44 / 42 MAP Kinase (phospho Thr202) Antibody
Product Specifications
Background
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) . Phosphorylates SPZ1
NCBI Gene ID
5595
Swiss Prot
P27361
Accession Number
NP_001035145.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
P44/42 MAP Kinase (Phospho-Thr202) antibody was raised against a peptide sequence around phosphorylation site of threonine 202 (F-L-T (p) -E-Y) derived from Human p44/42 MAP Kinase.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific, Apoptosis, Cancer
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
42, 44 kDa
Specificity
This antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at threonine 202.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Symbol
MAPK3
NCBI Official Name
Mitogen-activated protein kinase 3
NCBI Organism
Homo sapiens
Background Reference 01
TETE HANNKEN, et al. (2000) Am Soc Nephrol 11:1387-1397Omar D. PerezNature et al. (2002) Biotechnology 20: 155 - 162 Jingui Yu, et al. (2005) Anesth Analg 101: 315-321Hironobu Ihn et al. (2000) Immunology 165: 2149-2155
Other Product Names
ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, PKD, PKD1, PRKCM, ERK, Extracellular signal- regulated kinase 1, Insulin-stimulated MAP2 kinase, MAP kinase 1
Tested Applications
WB, IHC, IF
Protein ID
91718897
Physical Properties
Liquid
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