BRCA1 (phospho Ser1423) Antibody

Product Specifications

Background

The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation

NCBI Gene ID

672

Swiss Prot

P38398

Accession Number

NP_009225.1

Host

Rabbit

Reactivity

Human

Immunogen

BRCA1 (Phospho-Ser1423) antibody was raised against a peptide sequence around phosphorylation site of serine 1423 (H-G-S (p) -Q-P) derived from Human BRCA1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

220 kDa

Specificity

This antibody detects endogenous level of BRCA1 only when phosphorylated at serine 1423.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

BRCA1

NCBI Official Name

Breast cancer 1, early onset

NCBI Organism

Homo sapiens

Background Reference 01

Kim ST, et al. (2002) Genes Dev; 16 (5) : 560-570Arlt MF, et al. (2004) Mol Cell Biol; 24 (15) : 6701-6709Zhang J, et al. (2004) Mol Cell Biol; 24 (2) : 708-718Greenberg RA, et al. (2006) Genes Dev; 20 (1) : 34-46Tibbetts RS, et al. (2000) Genes Dev; 14 (23) : 2989-3002