IRS 1 (phospho Ser639) Antibody

Product Specifications

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit

NCBI Gene ID

3667

Swiss Prot

P35568

Accession Number

NP_005535.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

IRS-1 (Phospho-Ser639) antibody was raised against a peptide sequence around phosphorylation site of serine 639 (P-K-S (p) -V-S) derived from Human IRS-1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

180 kDa

Specificity

This antibody detects endogenous level of IRS-1 only when phosphorylated at serine 639.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

IRS1

NCBI Official Name

Insulin receptor substrate 1

NCBI Organism

Homo sapiens

Background Reference 01

Ozes ON, et al. (2001) Proc Natl Acad Sci U S A; 98 (8) : 4640-4645Tzatsos A, et al. (2006) Mol Cell Biol; 26 (1) : 63-76Steppan CM, et al. (2005) Mol Cell Biol; 25 (4) : 1569-1575Batty IH, et al. (2004) Biochem J; 379 (Pt 3) : 641-651