4E BP1 (phospho Thr45) Antibody

Product Specifications

Background

4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

NCBI Gene ID

1978

Swiss Prot

Q13541

Accession Number

NP_004086.1

Host

Rabbit

Reactivity

Human

Immunogen

4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

18 kDa

Specificity

This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

EIF4EBP1

NCBI Official Name

Eukaryotic translation initiation factor 4E binding protein 1

NCBI Organism

Homo sapiens

Background Reference 01

Gingras AC, et al. (1998) Genes Dev 12 (4) : 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23) : 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5) : 1087-1097. Moody CA, et al. (2005) J Virol 79 (9) : 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4) : 1432-1437.