Beta Catenin (phospho Ser33) Antibody

Product Specifications

Background

Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.

NCBI Gene ID

1499

Swiss Prot

P35222

Accession Number

NP_001091679.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Beta-Catenin (Phospho-Ser33) antibody was raised against a peptide sequence around phosphorylation site of serine 33 (L-D-S (p) -G-I) derived from Human β -Catenin.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

92 kDa

Specificity

This antibody detects endogenous level of β -Catenin only when phosphorylated at serine 33.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CTNNB1

NCBI Official Name

Catenin (cadherin-associated protein), beta 1, 88kDa

NCBI Organism

Homo sapiens

Background Reference 01

Novak A, et al. (1998) Proc Natl Acad Sci U S A; 95 (8) : 4374-4379Marin O, et al. (2003) Proc Natl Acad Sci U S A; 100 (18) : 10193-10200Okamura H, et al. (2004) Mol Cell Biol; 24 (10) : 4184-4195Xing Y, et al. (2003) Genes Dev; 17 (22) : 2753-2764Barth AI, et al. (1999) Proc Natl Acad Sci U S A; 96 (9) : 4947-4952