IkB epsilon (phospho Ser22) Antibody

Product Specifications

Background

Inhibits NF-κ -B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-κ -B p50-p65 and p50-c-Rel complexes.

NCBI Gene ID

4794

Swiss Prot

O00221

Accession Number

NP_004547.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

IkB-epsilon (Phospho-Ser22) antibody was raised against a peptide sequence around phosphorylation site of serine 22 (I-E-S (p) -L-R) derived from Human IkB-ε .

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Apoptosis, Cancer

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

40 kDa

Specificity

This antibody detects endogenous level of IkappaB-ε only when phosphorylated at serine 22.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

NFKBIE

NCBI Official Name

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon

NCBI Organism

Homo sapiens

Background Reference 01

Shirane M, et al. (1999) J Biol Chem; 274 (40) : 28169-74Karin, M. and Ben-Neriah, Y. (2000) Annu. Rev. Immunol. ;18, 621-663. Chen, Z. J. et al. (1996) Cell; 84, 853-862. Brown, K. et al. (1995) Science; 267, 1485-1488.