P27Kip1 (phospho Thr187) Antibody

Product Specifications

Background

Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

NCBI Gene ID

1027

Swiss Prot

P46527

Accession Number

NP_004055.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

P27Kip1 (Phospho-Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

27 kDa

Specificity

This antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

CDKN1B

NCBI Official Name

Cyclin-dependent kinase inhibitor 1B (p27, Kip1)

NCBI Organism

Homo sapiens

Background Reference 01

Eguchi H, et al. (2003) Cancer Res; 63 (15) : 4739-46Le XF, et al. (2003) J Biol Chem; Connor MK, et al. (2003) ) Mol Biol Cell; 14 (1) : 201-13Fujita N, et al. (2002) J Biol Chem; 277 (32) : 28706-13Ishida N, et al. (2000) J Biol Chem; 275 (33) : 25146-54

Other Product Names

KIP1, MEN4, CDKN4, MEN1B, P27KIP1, MAPTL, MTBT1, TAU, CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1