MEK1 / MEK2 (phospho Ser217 / Ser221) Antibody
Product Specifications
Background
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
NCBI Gene ID
5604;5605
Swiss Prot
Q02750
Accession Number
NP_002746.1; NP_109587.1
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
MEK1/MEK2 (Phospho-Ser217/Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 217/221 (I-D-S (p) -M-A) derived from Human MEK1/MEK2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Phospho-Specific
Purification
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/mL
Buffer
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Modification
Phospho-Specific
Shipping Conditions
Blue Ice
Storage Conditions
Store antibody at -20˚ C for up to one year.
Calculated Molecular Weight
45 kDa
Specificity
This antibody detects endogenous level ofMEK1/MEK2 only when phosphorylated at serine 217/221.
Applications Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Symbol
MAP2K1 MAP2K2
NCBI Official Name
Mitogen-activated protein kinase kinase 1 / mitogen-activated protein kinase kinase 2
NCBI Organism
Homo sapiens
Background Reference 01
Shen R, et al. (2002) Mol Cell Biol; 22 (10) : 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4) : 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1) : 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11) : 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5) : 2304-2310
Other Product Names
CFC3, MEK1, MKK1, MAPKK1, PRKMK1, ERK activator kinase 1/ERK activator kinase2, MAP kinase kinase 1/MAP kinase kinase 2, MAP2K1/MAP2K1, MAPK/ERK kinase 1/MAPK/ERK kinase 2, MAPKK 1/MAPKK 1
Tested Applications
IHC, WB
Protein ID
5579478 / 13489054
Physical Properties
Liquid
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