MEK1 / MEK2 (phospho Ser217 / Ser221) Antibody

Product Specifications

Background

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

NCBI Gene ID

5604;5605

Swiss Prot

Q02750

Accession Number

NP_002746.1; NP_109587.1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

MEK1/MEK2 (Phospho-Ser217/Ser221) antibody was raised against a peptide sequence around phosphorylation site of serine 217/221 (I-D-S (p) -M-A) derived from Human MEK1/MEK2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

45 kDa

Specificity

This antibody detects endogenous level ofMEK1/MEK2 only when phosphorylated at serine 217/221.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

MAP2K1 MAP2K2

NCBI Official Name

Mitogen-activated protein kinase kinase 1 / mitogen-activated protein kinase kinase 2

NCBI Organism

Homo sapiens

Background Reference 01

Shen R, et al. (2002) Mol Cell Biol; 22 (10) : 3230-3236Preisinger C, et al. (2005) EMBO J; 24 (4) : 753-765Laine P, et al. (2000) Biochem J; 349 (Pt 1) : 19-25Yaglom J, et al. (2003) Mol Cell Biol; 23 (11) : 3813-3824Dahan S, et al. (2002) Infect Immun; 70 (5) : 2304-2310

Other Product Names

CFC3, MEK1, MKK1, MAPKK1, PRKMK1, ERK activator kinase 1/ERK activator kinase2, MAP kinase kinase 1/MAP kinase kinase 2, MAP2K1/MAP2K1, MAPK/ERK kinase 1/MAPK/ERK kinase 2, MAPKK 1/MAPKK 1