Bcr (phospho Tyr177) Antibody

Product Specifications

Background

GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.

NCBI Gene ID

613

Swiss Prot

P11274

Accession Number

NP_004318.3

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Bcr (Phospho-Tyr177) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 177 (P-F-Y (p) -V-N) derived from Human Bcr.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

210 kDa

Specificity

This antibody detects endogenous level of Bcr only when phosphorylated at tyrosine 177.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

BCR

NCBI Official Name

Breakpoint cluster region

NCBI Organism

Homo sapiens

Background Reference 01

Sattler M, et al. Mol Cell Biol. 1999 Nov; 19 (11) : 7473-7480. Li S et al. EMBO J. 2001 Dec 3; 20 (23) : 6793-6804. Million RP, et, al. Mol Cell Biol. 2004 Jun; 24 (11) : 4685-4695. Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907 – 30916.