SMC1 (phospho Ser957) Antibody

Product Specifications

Background

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

NCBI Gene ID

8243

Swiss Prot

Q14683

Accession Number

NP_006297.2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

SMC1 (Phospho-Ser957) antibody was raised against a peptide sequence around phosphorylation site of serine 957 (G-S-S (p) -Q-G) derived from Human SMC1.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Calculated Molecular Weight

145 kDa

Specificity

This antibody detects endogenous level of SMC1 only when phosphorylated at serine 957.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Symbol

SMC1A

NCBI Official Name

Structural maintenance of chromosomes 1A

NCBI Organism

Homo sapiens

Background Reference 01

Yang XM. et al. (2004) Mol Cell Biol. 24 (12) : 5290-5303. Kim ST. et al. (2002) Genes Dev. 16 (5) : 560-570. Revenkova E. et al. (2001) Mol Cell Biol. 21 (20) : 6984-6998