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HDAC2 (phospho Ser394) Antibody

Product Specifications

Background

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) . Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes

NCBI Gene ID

3066

Swiss Prot

Q92769

Accession Number

NP_001518.2

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

HDAC2 (Phospho-Ser394) antibody was raised against a peptide sequence around phosphorylation site of serine 394 (E-D-S (p) -G-D) derived from Human HDAC2.

Clonality

Polyclonal

Conjugation

Unconjugated

Type

Primary Antibodies

Field of Research

Phospho-Specific, Cell Cycle

Purification

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Concentration

1 mg/mL

Buffer

Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Modification

Phospho-Specific

Shipping Conditions

Blue Ice

Storage Conditions

Store antibody at -20˚ C for up to one year.

Product Datasheet

https://www.prosci-inc.com/?datasheet_sku=79-191

Calculated Molecular Weight

60 kDa

Specificity

This antibody detects endogenous level of HDAC2 only when phosphorylated at serine 394.

Applications Notes

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

Symbol

HDAC2

NCBI Official Name

Histone deacetylase 2

NCBI Organism

Homo sapiens

Background Reference 01

Tsai SC, et al. (2002) J Biol Chem; 277 (35) : 31826-33

Other Product Names

HD2, RPD3, YAF1, PRKCT, HD2

Tested Applications

WB, IHC, IF

Protein ID

116284376

Physical Properties

Liquid

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